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Tryptone Soy Broth (TSB) was obtained from Oxoid (Thermo Fisher Scientific, Australia), Enzymes: DNase-I was obtained from Invitrogen (Melbourne, Australia), α-Amylase from MP Biomedicals (NSW, Australia) and Proteinase K from Sigma-Aldrich (Sydney, Australia)

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Bruner et al (1993) exposed both male and female rats and mice to JP-4 for 6 hours per day, 5 days per week, over the course of 1 year, followed by a 12-month recovery period