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Nutr Cancer (2020) 72(8):1255–63
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After staining for cell surface antigens, the cells were fixed with fixation buffer (420801, Biolegend), permeabilized with permeabilization wash buffer (421002, Biolegend), and stained for intracellular antigens with anti-mouse APC FoxP-3 (320011, Biolegend), FITC Granzyme B (515403, Biolegend), and APC IFNγ (505809, Biolegend)