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RNA samples were treated with the TURBO DNA-free kit (Applied Biosystems, Foster City, CA), further purified through an RNease kit (Qiagen) and quantified with a NanoDrop-1000 (NanoDrop Technologies Inc., Wilmington, DE)

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Dysbiosis may be one of the environmental factors contributing to the etiology of GERD (23) ( Figure 1 )

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