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The chromatographic column can be a C 18 chromatographic column (Kromasil-C 18 , 30mm*250mm, Hanbang Technology Chromatography Co., Ltd.), and the samples are loaded in multiple times, and the effluents of the target peaks are collected respectively
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Size:50 Grams
Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]
Copper peptide
